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Bio Molecular Systems bio molecular systems mic qpcr cycler
Bio Molecular Systems Mic Qpcr Cycler, supplied by Bio Molecular Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio+molecular+systems+mic+qpcr+cycler/pm39428081-154-5-5
Average 96 stars, based on 1 article reviews
bio molecular systems mic qpcr cycler - by Bioz Stars, 2026-09
96/100 stars

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Real-time Polymerase Chain Reaction:

Article Title: Development of isoselenazolium chlorides as selective pyruvate kinase isoform M2 inhibitors.
Article Snippet: Alterations in cancer metabolic pathways open up an opportunity for targeted and effective elimination of tumor cells.. Pyruvate kinase M2 (PKM2) is predominantly expressed in proliferating cells and plays an essential role in directing glucose metabolism in cancer.. Here, we report the design of novel class of selective PKM2 inhibitors as anti-cancer agents and their mechanism of action.

Article Title: Test performance study for detection of Ralstonia solanacearum and Clavibacter sepedonicus in potato tubers with TaqMan PCR
Article Snippet: R. A. M. Vreeburg , M. Nas , B.. De Paepe , T. Dreo , R. A. Gottsberger , E. Fornefeld , K. Fraser , S. Leonard , A. C. Le Roux , E. T. M. Meekes , C. Rivoal , L. Smits-Mastebroek and J. van Vaerenbergh Nederlandse Algemene Keuringsdienst (NAK), Emmeloord (the Netherlands); e-mail: r.vreeburg@nak.nl Institute for Agriculture, Fisheries and Food Research (ILVO), Centre for Diagnosis of Plant Pests – Bacteriology, Merelbeke (Belgium) Department of Biotechnology and Systems Biology, National Institute of Biology (NIB), Ljubljana (Slovenia) Department for Molecular Diagnostics of Plant Diseases, Austrian Agency for Health and Food Safety (AGES), Institute for Sustainable Plant Production, Vienna (Austria) Julius K€ uhn Institut Institute (JKI), Institute for National and International Plant Health, Braunschweig (Germany) Diagnostics, Wildlife & Molecular Biology, Science and Advice for Scottish Agriculture (SASA), Edinburgh (UK) Section Phytopathologie, Laboratoire F ed eral pour la S ecurit e de la Chaı̂ne Alimentaire, AFSCA, Gembloux, (Belgium) INRA, Resistance and Adaptation, Rennes (France) Naktuinbouw, Roelofsarendsveen (the Netherlands) Anses – Laboratoire de la Sant e des V eg etaux, Equipe Bact eriologie, Angers (France)

Article Title: Compositions and methods for the prevention and/or treatment of mitochondrial disease, including Friedreich's ataxia
Article Snippet: After incubation, cell media was removed and total RNA from the cells was isolated using E.Z.N.A.® Total RNA Kit I (Omega Bio-tek, Norcross, Georgia, USA) spin columns, and first-strand cDNA synthesis was carried out using a High Capacity cDNA Reverse Transcription Kit (Applied BiosystemsTM, Foster City, CA) following the manufacturer's instructions. qPCR analysis of gene expression was performed by mixing SYBR® Green Master Mix (Applied BiosystemsTM) with synthesized cDNA and forward and reverse primers. .. Reactions were run on a Bio-Molecular Systems MIC qPCR Cycler according to the manufacturer's protocol and using the following conditions: 95° C. for 10 min, [95° C. for 15 seconds, 60° C. for 60 seconds] (60 cycles), and 95° C. for 60 seconds, followed by melt curve analysis at 72-95° C., 0.3° C./sec. .. Reactions were run on a Bio-Molecular Systems MIC qPCR Cycler according to the manufacturer's protocol and using the following conditions: 95° C. for 10 min, [95° C. for 15 seconds, 60° C. for 60 seconds] (60 cycles), and 95° C. for 60 seconds, followed by melt curve analysis at 72-95° C., 0.3° C./sec.

Article Title: Age-dependent changes in visceral adiposity are associated with decreased plasma levels of DHEA-S in sigma-1 receptor knockout male mice.
Article Snippet: .. Reactions were run on a Bio-Molecular Systems MIC qPCR Cycler according to the manufacturer's protocol. ..

Article Title: Compositions and methods for the prevention and/or treatment of mitochondrial disease, including Friedreich's ataxia
Article Snippet: .. Reactions were run on a Bio-Molecular Systems MIC qPCR Cycler according to the manufacturer's protocol and using the following conditions: 95° C. for 10 min, [95° C. for 15 seconds, 60° C. for 60 seconds] (60 cycles), and 95° C. for 60 seconds, followed by melt curve analysis at 72-95° C., 0.3° C./sec. ..

Gene Expression:

Article Title: Development of isoselenazolium chlorides as selective pyruvate kinase isoform M2 inhibitors.
Article Snippet: Alterations in cancer metabolic pathways open up an opportunity for targeted and effective elimination of tumor cells.. Pyruvate kinase M2 (PKM2) is predominantly expressed in proliferating cells and plays an essential role in directing glucose metabolism in cancer.. Here, we report the design of novel class of selective PKM2 inhibitors as anti-cancer agents and their mechanism of action.

SYBR Green Assay:

Article Title: Development of isoselenazolium chlorides as selective pyruvate kinase isoform M2 inhibitors.
Article Snippet: Alterations in cancer metabolic pathways open up an opportunity for targeted and effective elimination of tumor cells.. Pyruvate kinase M2 (PKM2) is predominantly expressed in proliferating cells and plays an essential role in directing glucose metabolism in cancer.. Here, we report the design of novel class of selective PKM2 inhibitors as anti-cancer agents and their mechanism of action.



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Key resource table

Journal: Cell reports

Article Title: Widespread sex-dimorphism across single-cell transcriptomes of adult African turquoise killifish tissues

doi: 10.1016/j.celrep.2023.113237

Figure Lengend Snippet: Key resource table

Article Snippet: 85 https://cran.r-project.org RUVSeq v1.20.0 Risso et al 86 https://github.com/drisso/RUVSeq scds v1.2.0 Bais et al 87 https://github.com/kostkalab/scds scProportionTest v0.0.0.9000 Miller et al 54 https://github.com/rpolicastro/scProportionTest scSorter v0.0.2 Guo et al 88 https://github.com/cran/scSorter SCTransform v0.3.2 Hafemeister et al 89 https://github.com/satijalab/sctransform scType 1.0 Ianevski et al 90 https://github.com/IanevskiAleksandr/sc-type scvelo v0.2.5 Bergen et al 91 https://github.com/theislab/scvelo Seurat v3.2.2 Satija et al, Butler et al, Stuart et al, Hao et al 92 – 95 https://github.com/satijalab/seurat/releases/tag/v3.2.2 SingleCellNet v0.1.0 Tan et al 96 https://github.com/pcahan1/singleCellNet velocyto v0.17.17 La Manno et al 44 https://github.com/velocyto-team/velocyto.py Other 10x Genomics User Guide Chromium Next GEM Single-cell 3′ Reagent Kits v3.1 10x Genomics Cat# CG000204 , Rev D 4200 TapeStation system Agilent Technologies Cat# G2991A 5424R Microcentrifuge Eppendorf Cat# 05-401-203 63x/1.4 Plan ApoChromat Oil DIC M27 Objective Zeiss Cat# 420782-9900-000 Beadbug 6 microtube homogenizer Benchmark Scientific Cat# D1036 Chromium Next GEM Chip G 10x Genomics Cat# 2000177 Chromium Next GEM Single Cell 3’ GEM, Library & Gel Bead Kit v3.1 10x Genomics Cat# PN-1000121 Countess Cell Counting Chamber Slides Thermo Fisher Scientific Cat# C10228 Countess II FL Automated Cell Counter Thermo Fisher Scientific Cat# AMQAX1000 Cryostat CM 1860 Leica Cat# 14-0491-46884 EasySep Dead Cell Removal (Annexin V) Kit STEMCELL Technologies Cat# 17899 Keyence BZ-X710 Keyence Unavailable Liver Dissociation Kit Miltenyi Biotec Cat# 130-105-807 Low Retention Pipette Tips USA Scientific Cat# 1183-1710 Lysing Matrix D tube MP Cat# 6913500 MACSQuant Analyzer 10 Miltenyi Biotec Cat#130-096-343 Magnetic Induction Cycler (MIC) machine Bio Molecular Systems Cat# MIC-2 PTFE-coated microtome blades Duraedge Cat# 7223 Qubit ® 3.0 Fluorometer Thermo Fisher Scientific Cat# Q33216 RNAscope ™ Multiplex Fluorescent Reagent Kit v2 With Sample Preparation and Pretreatment User Guide Advanced Cell Diagnostics UM323100 SensiFAST SYBR ® No-ROX Kit Bioline Cat# BIO-98020 Stellaris 5 Confocal Microscope Leica NA Superfrost Plus Micro Slides VWR Cat# 48311-703 Thermal cycler C1000 Bio-Rad Cat# 1851196 Open in a separate window Key resource table

Techniques: Recombinant, Modification, RNAscope, Staining, Quantitation Assay, Sensitive Assay, Multiplex Assay, Control, Microscopy, Software, Cell Counting, Transferring, Sample Prep